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Effect of berbamine on 5-ALA-induced PpIX accumulation. <t>RT112,</t> RT112 LTT, RT112 CSC (top), and J82, J82 LTT, J82 CSC (bottom) were treated with 5-ALA (0–100 µg/ml) and berbamine (no, ½ IC 50 , IC 50 ). PpIX fluorescence intensity was normalized to cell viability and is shown as individual values with mean as a line. Asterisks indicate significance compared to the sample without berbamine (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001).
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Effect of berbamine on 5-ALA-induced PpIX accumulation. <t>RT112,</t> RT112 LTT, RT112 CSC (top), and J82, J82 LTT, J82 CSC (bottom) were treated with 5-ALA (0–100 µg/ml) and berbamine (no, ½ IC 50 , IC 50 ). PpIX fluorescence intensity was normalized to cell viability and is shown as individual values with mean as a line. Asterisks indicate significance compared to the sample without berbamine (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001).
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Effect of berbamine on 5-ALA-induced PpIX accumulation. <t>RT112,</t> RT112 LTT, RT112 CSC (top), and J82, J82 LTT, J82 CSC (bottom) were treated with 5-ALA (0–100 µg/ml) and berbamine (no, ½ IC 50 , IC 50 ). PpIX fluorescence intensity was normalized to cell viability and is shown as individual values with mean as a line. Asterisks indicate significance compared to the sample without berbamine (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001).
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Effect of berbamine on 5-ALA-induced PpIX accumulation. <t>RT112,</t> RT112 LTT, RT112 CSC (top), and J82, J82 LTT, J82 CSC (bottom) were treated with 5-ALA (0–100 µg/ml) and berbamine (no, ½ IC 50 , IC 50 ). PpIX fluorescence intensity was normalized to cell viability and is shown as individual values with mean as a line. Asterisks indicate significance compared to the sample without berbamine (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001).
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Effect of berbamine on 5-ALA-induced PpIX accumulation. <t>RT112,</t> RT112 LTT, RT112 CSC (top), and J82, J82 LTT, J82 CSC (bottom) were treated with 5-ALA (0–100 µg/ml) and berbamine (no, ½ IC 50 , IC 50 ). PpIX fluorescence intensity was normalized to cell viability and is shown as individual values with mean as a line. Asterisks indicate significance compared to the sample without berbamine (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001).
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Effect of berbamine on 5-ALA-induced PpIX accumulation. <t>RT112,</t> RT112 LTT, RT112 CSC (top), and J82, J82 LTT, J82 CSC (bottom) were treated with 5-ALA (0–100 µg/ml) and berbamine (no, ½ IC 50 , IC 50 ). PpIX fluorescence intensity was normalized to cell viability and is shown as individual values with mean as a line. Asterisks indicate significance compared to the sample without berbamine (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001).
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Effect of berbamine on 5-ALA-induced PpIX accumulation. <t>RT112,</t> RT112 LTT, RT112 CSC (top), and J82, J82 LTT, J82 CSC (bottom) were treated with 5-ALA (0–100 µg/ml) and berbamine (no, ½ IC 50 , IC 50 ). PpIX fluorescence intensity was normalized to cell viability and is shown as individual values with mean as a line. Asterisks indicate significance compared to the sample without berbamine (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001).
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Effect of berbamine on 5-ALA-induced PpIX accumulation. RT112, RT112 LTT, RT112 CSC (top), and J82, J82 LTT, J82 CSC (bottom) were treated with 5-ALA (0–100 µg/ml) and berbamine (no, ½ IC 50 , IC 50 ). PpIX fluorescence intensity was normalized to cell viability and is shown as individual values with mean as a line. Asterisks indicate significance compared to the sample without berbamine (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001).

Journal: Scientific Reports

Article Title: Combining 5-ALA-PDT with berbamine as an in vitro multimodal therapy approach against bladder cancer cells

doi: 10.1038/s41598-026-46092-x

Figure Lengend Snippet: Effect of berbamine on 5-ALA-induced PpIX accumulation. RT112, RT112 LTT, RT112 CSC (top), and J82, J82 LTT, J82 CSC (bottom) were treated with 5-ALA (0–100 µg/ml) and berbamine (no, ½ IC 50 , IC 50 ). PpIX fluorescence intensity was normalized to cell viability and is shown as individual values with mean as a line. Asterisks indicate significance compared to the sample without berbamine (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001).

Article Snippet: RT112 (DSMZ, cat. no. ACC-418) and J82 (ATCC, cat. no. HTB-1) cell lines originate from transitional cell carcinoma of the human bladder.

Techniques: Fluorescence

Cell viability of RT112, RT112 LTT and RT112 CSC following 5-ALA-PDT treatment with increasing irradiation doses and varying concentrations of 5-ALA and berbamine. Bars represent weighted means ± SD from three independent experiments ( n = 3 × 6; controls without irradiation n = 3 × 36). Asterisks indicate significance of unweighted means compared to the irradiated control sample without 5-ALA C1.1-C1.4 (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001).

Journal: Scientific Reports

Article Title: Combining 5-ALA-PDT with berbamine as an in vitro multimodal therapy approach against bladder cancer cells

doi: 10.1038/s41598-026-46092-x

Figure Lengend Snippet: Cell viability of RT112, RT112 LTT and RT112 CSC following 5-ALA-PDT treatment with increasing irradiation doses and varying concentrations of 5-ALA and berbamine. Bars represent weighted means ± SD from three independent experiments ( n = 3 × 6; controls without irradiation n = 3 × 36). Asterisks indicate significance of unweighted means compared to the irradiated control sample without 5-ALA C1.1-C1.4 (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001).

Article Snippet: RT112 (DSMZ, cat. no. ACC-418) and J82 (ATCC, cat. no. HTB-1) cell lines originate from transitional cell carcinoma of the human bladder.

Techniques: Irradiation, Control

Berbamine has cytotoxic effects in ROS generation, cell migration and apoptosis rate for cisplatin-resistant cell lines RT112 LTT and J82 LTT. Tert-butyl hydroperoxide (TBHP; 150 µM) served as positive control for determination of reactive oxygen species (ROS). Detailed data presentation for all cell lines are available in the supplementary section (Supplementary Figs. , and ). Data represent mean ± SD ( n = 3). Asterisks indicate significance relative to the control without berbamine (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001).

Journal: Scientific Reports

Article Title: Combining 5-ALA-PDT with berbamine as an in vitro multimodal therapy approach against bladder cancer cells

doi: 10.1038/s41598-026-46092-x

Figure Lengend Snippet: Berbamine has cytotoxic effects in ROS generation, cell migration and apoptosis rate for cisplatin-resistant cell lines RT112 LTT and J82 LTT. Tert-butyl hydroperoxide (TBHP; 150 µM) served as positive control for determination of reactive oxygen species (ROS). Detailed data presentation for all cell lines are available in the supplementary section (Supplementary Figs. , and ). Data represent mean ± SD ( n = 3). Asterisks indicate significance relative to the control without berbamine (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001).

Article Snippet: RT112 (DSMZ, cat. no. ACC-418) and J82 (ATCC, cat. no. HTB-1) cell lines originate from transitional cell carcinoma of the human bladder.

Techniques: Migration, Positive Control, Control